Iranian Journal of War and Public Health

eISSN (English): 2980-969X
eISSN (Persian): 2008-2630
pISSN (Persian): 2008-2622
Volume 15, Issue 2 (2023)                   Iran J War Public Health 2023, 15(2): 199-205 | Back to browse issues page

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Shnaien A, Mohammad A, Hassan E. Neuroprotective Effects of Semaglutide in Endotoxemia Mouse Model. Iran J War Public Health 2023; 15 (2) :199-205
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1- Department of Pharmacology & Therapeutics, Faculty of Medicine, University of Kufa, Najaf, Iraq
* Corresponding Author Address: Department of Pharmacology & Therapeutics, Faculty of Medicine, University of Kufa, Kufa Street, Najaf, Iraq. Postal Code: P. O. Box 21, Najaf, Iraq. (
Abstract   (832 Views)
Aims: The present study aimed to examine the neuroprotective effects of semaglutide during endotoxemia and its role in modulating pro-inflammatory mediators.
Materials & Methods: Twenty-four adult male Swiss albino mice, 8-12 weeks old, weighing 25-35g, were randomly divided into four equal groups (n=6), including sham (laparotomy without cecal ligation and puncture, sepsis (laparotomy with CLP), vehicle (equivalent volume of distilled water before CLP), and semaglutide (40µg/kg/day before CLP). The brain was used for tissue evaluation of TNF-α, IL-6, IL-1β, TLR4, and P-STAT3, as well as for histological examination.
Findings: The tissue levels of TNF-α, IL-6 and IL-in the sham group were significantly lower than the sepsis and vehicle groups (p<0.05). In the semaglutide group, tissue levels of TNF-α, IL-6, and IL-were significantly lower than the sepsis and vehicle groups (p<0.05). The tissue levels of TLR4 and STAT3 in the sham group were significantly lower than the sepsis and vehicle groups (p<0.05). Also, tissue levels of TLR4 and STAT3 in the semaglutide group were significantly lower than the sepsis and vehicle groups (p<0.05). Histopathologically, semaglutide considerably reduced brain damage compared to the sepsis and vehicle groups.
Conclusion: Semaglutide can reduce brain dysfunction during CLP-induced polymicrobial sepsis in male mice through its modulating effects on TLR4STAT3 downstream signaling pathways and subsequently reducing inflammatory cytokines TNF-α, IL-6, and IL-1β.

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