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Iran J War Public Health 2023, 15(1): 1001-1011 Back to browse issues page
Protective Effects of Dulaglutide On Lung Injury in Endotoxemia Mouse Model
Aqeel Qays Abd Uljaleel1, Ekhlas Sabah Hassan *2, Ammar Rasoul Mohammad1, Najah Raish Hadi1
1- Department of Pharmacology & Therapeutics, Faculty of Medicine, University of Kufa
2- Department of Pharmacology & Therapeutics, Faculty of Medicine, University of Kufa , ekhlass.khazaal@uokufa.edu.iq
Abstract:   (30 Views)
Background: Endotoxemia is the most common condition in patients treated in critical care units. Septic shock is characterized multiple organ dysfunction, acute respiratory distress syndrome (ARDS), Acute Lung Injury (ALI), and high mortality.to investigate if dulaglutide may help to protect the lungs during endotoxemia by modulating the inflammatory and oxidative stress pathways.
Materials & Methods: 20 adult male Swiss-albino mice aged 9–12 weeks, weighted 25–35g, were randomized into four equal groups (n=5), sham group (laparotomy without ceacal ligation and puncture (CLP), CLP group (laparotomy with CLP), vehicle group (normal saline 2 weeks prior to CLP), and Dulaglutide group (0.6mg/kg twice weekly S.C for 2 weeks prior to CLP).
After 24 hrs of sepsis, the lung tissue harvested and used to assess IL-6, IL-1β, TNF-α, MIF, TLR4 and F2-isoprostane as well as histological examination.
Findings: Lung tissue levels of IL-6, IL-1β, TNF-α, MIF, TLR4 and F2-isoprostane were significantly higher in sepsis group (p<0.05) as compared with sham group, while dulaglutide group showed significant (p<0.05) lower level in these inflammatory mediators and oxidative stress as compared to sepsis group. Histologically, all mice in sepsis group showed a significant (p<0.05) lung tissue injury, but this injury was significantly (p<0.05) reduced in dulaglutide pre-treated group.
Conclusion: dulaglutide has the ability to attenuate acute lung injury during CLP induced endotoxemia in mice through its modulating effects on TLR4 and oxidative stress, downstream signalling pathways and subsequently decreased lung tissue levels of pro-inflammatory mediaters.
 
Keywords: Dulaglutide, CLP, endotoxemia, TLR4, F2-isoprostane
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Article Type: Original Research | Subject: Diagnosis and Treatment of Veterans or Handicapped Diseases
Received: 2022/12/27 | Accepted: 2023/02/20 | Published: 2023/03/11
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Abd Uljaleel A Q, Hassan E S, Mohammad A R, Hadi N R. Protective Effects of Dulaglutide On Lung Injury in Endotoxemia Mouse Model. Iran J War Public Health 2023; 15 (1) :1001-1011
URL: http://ijwph.ir/article-1-1289-en.html


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Volume 15, Issue 1 (2023) Back to browse issues page
مجله طب جانباز Iranian Journal of War and Public Health
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