Received: 2014/07/26 | Accepted: 2014/08/19 | Published: 2015/01/25
URL: http://ijwph.ir/article-1-428-en.html
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2- Immunoregulation Research Center, Shahed University, Tehran, Iran , tghazanfari@yahoo.com
3- Zist Kavosh Iranian (IBRESCO), Tehran, Iran
Aims: Mustard gas is an Alkalizing agent and
inflammatory stimuli that were used as chemical weapon. Lung problems in people
exposed to mustard gas is the major cause of morbidity and mortality in
long-term. DNA damage is considered as one of the most important clinical
lesions. Typically, apoptosis is occurred during development and aging and as a
hemostatic mechanism for maintaining cell populations in tissues and also as a
defence mechanism in immune system body reactions or when cells are suffered by
toxic agents or illness. The aim of this study was to compare the rate of apoptosis in blood
mononuclear cells of chemical victims 25 years after exposure to mustard gas
and non-exposured people.
Materials & Methods: This case control study was performed at 2014 on
10 veterans as case group selected by random sampling and 11 patients as control group selected by random
sampling. 3mL of peripheral blood collected from both groups and
gradient Faykl was used to isolation of mononuclear peripheral blood cells. After cells
preparing, ELISA kit cell death was used to measure apoptosis. Data were
analysed by student-T test.
Findings: The mean incidence of apoptosis in control group was 0.533±0.168 and in
case group was 0.345±0.116, which was not different significantly (p=0.39).
Conclusion: Mustard gas in chronic phase (25 years after exposure) has no effect on apoptosis of peripheral blood mononuclear cells.