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Volume 15, Issue 4 (2023)
Iran J War Public Health 2023, 15(4): 387-394 |
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Received: 2023/07/5 | Accepted: 2023/12/10 | Published: 2023/12/19
Received: 2023/07/5 | Accepted: 2023/12/10 | Published: 2023/12/19
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Emaduldeen H, Waheeb M, Makki M. Antimicrobial Features of Lepidium sativum L. extract. Iran J War Public Health 2023; 15 (4) :387-394
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1- Environment Research Center, University of Technology, Baghdad, Iraq
2- Department of Biology, College of Science, Al Muthanna University, AL Muthanna, Iraq
2- Department of Biology, College of Science, Al Muthanna University, AL Muthanna, Iraq
Abstract (874 Views)
Aims: Garden cress (Lepidium sativum L.), a member of the Cruciferae family, is widely planted worldwide, especially in India, Europe, and the United States. One of the most common mosquito species that carries Plasmodium falciparum is the Anopheles gambiae sensu lato, which may be combated by sprinkling fields with Lepidium sativum seeds. This study aimed to test the Lepidium sativum Linn. seeds extract as an antimicrobial agent against food pathogens.
Materials & Methods: The active components were extracted from the powdered dry seeds using chloroform, ethyl acetate, methanol, and dichloromethane. The antibacterial activity of various doses of the extracts was evaluated using agar well diffusion. The MIC and MBC for the most effective extract were estimated using tube dilution and subculturing methods.
Findings: Staphylococcus aureus had an MIC of 1.56mg/ml and a minimum inhibitory concentration (MBC) of 6.52mg/ml, while Salmonella typhi had an MIC of 25mg/ml. At a concentration of 10% L. sativum, the half-life (KDT50) was determined to be 7.34 minutes. Conversely, the half-life of L. sativum oil was only 10.05 minutes when used at the same concentration. Compared to the L. sativum concentrations that resulted in the KDT50 (50 and 10%, respectively), the bendiocarb 0.1% impregnated filter paper performed poorly.
Conclusion: L. sativum essential oil effectively kills Anopheles gambiae.
Materials & Methods: The active components were extracted from the powdered dry seeds using chloroform, ethyl acetate, methanol, and dichloromethane. The antibacterial activity of various doses of the extracts was evaluated using agar well diffusion. The MIC and MBC for the most effective extract were estimated using tube dilution and subculturing methods.
Findings: Staphylococcus aureus had an MIC of 1.56mg/ml and a minimum inhibitory concentration (MBC) of 6.52mg/ml, while Salmonella typhi had an MIC of 25mg/ml. At a concentration of 10% L. sativum, the half-life (KDT50) was determined to be 7.34 minutes. Conversely, the half-life of L. sativum oil was only 10.05 minutes when used at the same concentration. Compared to the L. sativum concentrations that resulted in the KDT50 (50 and 10%, respectively), the bendiocarb 0.1% impregnated filter paper performed poorly.
Conclusion: L. sativum essential oil effectively kills Anopheles gambiae.
Keywords:
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