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Erythromycin resistance genes among coagulase negative staphylococci isolated from human in Basrah, Iraq
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| Jean M. Mezban, Basil A. Abbas, Mohammed H. Khudor * |
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Abstract: (37 Views) |
The samples used in this study were obtained from human at different areas of Basrah province. 80 sample collected from human including 40 samples from human hand swabs and 40 from nasal swabs. Results showed that only 37 (46.25%) positive for staphylococcal infection distributed 19 (46.25%) and18 (45%) from human hand swabs and from nasal swabs respectively. Statistical analysis shows that there is no significant difference p value <0.01 between the type of sample and isolates number. There were 5 species of CNS according to Vitek 2 kit. Staphylococci fall in following species, S. sciuri, S. lentus, S. gallinarum, S. chromogen, S. haemolyticus. Staphylococci were examined for their susceptibility to different antibiotics and found to be resistance to several of them. Polymerase Chain Reaction (PCR) was done for the detection of Erm(A) gen region in 20 isolates of Staphylococcus, out of 20 sample the amplification cleared only 12 sample positive for Erm(A) gen region with PCR product 190 bp. Results showed also the presence of ErmC gene with a clear band with size of 299 bp, which represents the correct expected band in 8 isolates from all the subjected isolates.
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| Keywords: Erythromycin, Resistant genes, CoNS, Human, PCR |
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Article Type: Original Research |
Subject:
Diagnosis and Treatment of Veterans or Handicapped Diseases
Received: 2022/06/29 | Accepted: 2023/01/30 | Published: 2023/03/1
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