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Volume 17, Issue 2 (2025)
Iran J War Public Health 2025, 17(2): 1001-1012 |
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Ethics code: IR.UM.REC.1402.276
History
Received: 2025/05/13 | Accepted: 2025/05/25 | Published: 2025/04/21
Received: 2025/05/13 | Accepted: 2025/05/25 | Published: 2025/04/21
How to cite this article
Allami M, Majeed Al-Shammari A, Neshati Z. Generation and Characterization of Purkinje Like Cells from Differentiated Mouse Bone Marrow Mesenchymal Stem Cells. Iran J War Public Health 2025; 17 (2) :1001-1012
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1- Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran
2- Experimental Therapy Department, Iraqi Center for Cancer and Medical Genetic Research, Mustansiriyah University
3- Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran2Novel Diagnostics and Therapeutics Research Group, Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran ,neshati@um.ac.ir
2- Experimental Therapy Department, Iraqi Center for Cancer and Medical Genetic Research, Mustansiriyah University
3- Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran2Novel Diagnostics and Therapeutics Research Group, Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran ,
Abstract (89 Views)
Aims Neurological disorders remain difficult to treat due to the central nervous system’s limited capacity for neuronal regeneration. Mesenchymal stem cells (MSCs) offer a promising approach for neuron replacement therapies. This study aimed to establish a novel protocol for differentiating MSCs into Purkinje-like cells, given the critical role of Purkinje neurons in motor coordination, cognition, and emotional regulation.
Instrument & Methods MSCs were isolated from the bone marrow of mice and characterized using specific markers (CD34, CD44, CD45, and CD73). Then, the MSCs were differentiated into neural stem cells and characterized by immunocytochemistry and neurosphere formation (Nestin and SOX2). Finally, neural stem cells were differentiated into Purkinje-like cells via specific culture media and identified by morphology and immunocytochemistry (calbindin D28K and IP3R1).
Findings According to the International Society for Cell and Therapy criteria, isolated MSCs adhered to the flask surface and expressed the CD73 and CD44 markers but did not express the CD34 and CD45 markers. The identity of neural stem cells was confirmed through their morphology, expression of Nestin and SOX2 markers, and neurosphere formation. Calbindin D28K and IP3R1 markers were significantly expressed in Purkinje-like cells differentiated from MSCs.
Conclusion Given the broad need for innovative strategies in neural repair, the generation of Purkinje-like neurons from readily accessible stem cells presents a promising therapeutic avenue for treating neurological disorders. Beyond its general applications in public health, this approach holds particular potential for improving outcomes in patients affected by brain injuries, including those resulting from war-related trauma.
Instrument & Methods MSCs were isolated from the bone marrow of mice and characterized using specific markers (CD34, CD44, CD45, and CD73). Then, the MSCs were differentiated into neural stem cells and characterized by immunocytochemistry and neurosphere formation (Nestin and SOX2). Finally, neural stem cells were differentiated into Purkinje-like cells via specific culture media and identified by morphology and immunocytochemistry (calbindin D28K and IP3R1).
Findings According to the International Society for Cell and Therapy criteria, isolated MSCs adhered to the flask surface and expressed the CD73 and CD44 markers but did not express the CD34 and CD45 markers. The identity of neural stem cells was confirmed through their morphology, expression of Nestin and SOX2 markers, and neurosphere formation. Calbindin D28K and IP3R1 markers were significantly expressed in Purkinje-like cells differentiated from MSCs.
Conclusion Given the broad need for innovative strategies in neural repair, the generation of Purkinje-like neurons from readily accessible stem cells presents a promising therapeutic avenue for treating neurological disorders. Beyond its general applications in public health, this approach holds particular potential for improving outcomes in patients affected by brain injuries, including those resulting from war-related trauma.
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