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Volume 17, Issue 2 (2025)
Iran J War Public Health 2025, 17(2): 1001-1008 |
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Ethics code: INTI-FHLS-03-01-2021
History
Received: 2025/02/26 | Accepted: 2025/04/27 | Published: 2025/04/21
Received: 2025/02/26 | Accepted: 2025/04/27 | Published: 2025/04/21
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Raghad A. Allami, Ali M. Mutlag. Correlation Between HMGB1 Gene Expression and CCK Hormone Levels in Patients with Gallbladder Disorders. Iran J War Public Health 2025; 17 (2) :1001-1008
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Authors
Raghad A. Allami *1, Ali M. Mutlag2
1- Department of Pathological Analyses, College of Sciences, University of Wasit, Iraq , raghad.hussien@uowasit.edu.iq
2- Department of Pathological Analyses, College of Science, University of Wasit, Iraq
2- Department of Pathological Analyses, College of Science, University of Wasit, Iraq
Abstract (279 Views)
Objective: This study aimed to evaluate the association between High-mobility group protein 1(HMGB1) gene expression and cholecystokinin (CCK) levels in patients with gallbladder disease before and after cholecystectomy.
Methods: This study was conducted at the College of Science, Wasit University, Iraq. Patient samples were collected from Al Karama Teaching Hospital and Al Zahraa Hospital between October 2023 to July 2024 The study included (40) patients with cholecystitis who were divided into two categories (40 patients) pre- surgery and (20) continuance as post-surgery samples, along with 20 healthy controls. with an age range of 20–79 years. Serum CCK levels were measured using ELISA, whereas HMGB1 gene expression was analyzed using real-time PCR.
Results: The results indicated a significant increase in serum CCK concentration across all study groups (P < 0.05), with no significant difference between postsurgical patients and healthy controls (P > 0.05). HMGB1 gene expression was significantly higher in pre-surgical patients (4.91 ± 0.51) compared to post-surgical patients (1.95 ± 0.24) and healthy controls (1.0) (P < 0.05). ROC analysis revealed that CCK had an area under the curve (AUC) of 0.717 (P = 0.002), with 72.5% sensitivity, while HMGB1 gene expression showed excellent diagnostic accuracy with an AUC of 0.950 (P = 0.001) and 95.0% sensitivity. Statistical analysis also showed a significant positive correlation between HMGB1 gene expression and CCK levels both before (r = 0.323, P = 0.042) and after surgery (r = 0.419, P = 0.033).
Conclusion: This study suggests that HMGB1 gene expression is significantly associated with gallbladder disease progression, with levels decreasing after cholecystectomy. This is the first study to observe a correlation between HMGB1 and CCK, indicating a potential interaction between inflammation and gallbladder motility. These findings highlight HMGB1 as a potential biomarker for gallbladder disease and postsurgical recovery.
Methods: This study was conducted at the College of Science, Wasit University, Iraq. Patient samples were collected from Al Karama Teaching Hospital and Al Zahraa Hospital between October 2023 to July 2024 The study included (40) patients with cholecystitis who were divided into two categories (40 patients) pre- surgery and (20) continuance as post-surgery samples, along with 20 healthy controls. with an age range of 20–79 years. Serum CCK levels were measured using ELISA, whereas HMGB1 gene expression was analyzed using real-time PCR.
Results: The results indicated a significant increase in serum CCK concentration across all study groups (P < 0.05), with no significant difference between postsurgical patients and healthy controls (P > 0.05). HMGB1 gene expression was significantly higher in pre-surgical patients (4.91 ± 0.51) compared to post-surgical patients (1.95 ± 0.24) and healthy controls (1.0) (P < 0.05). ROC analysis revealed that CCK had an area under the curve (AUC) of 0.717 (P = 0.002), with 72.5% sensitivity, while HMGB1 gene expression showed excellent diagnostic accuracy with an AUC of 0.950 (P = 0.001) and 95.0% sensitivity. Statistical analysis also showed a significant positive correlation between HMGB1 gene expression and CCK levels both before (r = 0.323, P = 0.042) and after surgery (r = 0.419, P = 0.033).
Conclusion: This study suggests that HMGB1 gene expression is significantly associated with gallbladder disease progression, with levels decreasing after cholecystectomy. This is the first study to observe a correlation between HMGB1 and CCK, indicating a potential interaction between inflammation and gallbladder motility. These findings highlight HMGB1 as a potential biomarker for gallbladder disease and postsurgical recovery.
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