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Iran J War Public Health 2022, 14(2): 139-146 Back to browse issues page
Antibacterial Activity of Capsicum annum L. Juice Against Klebsiella pneumonia Isolated from Respiratory Tract Infections
B. Shakir Abed Almjalawi *1, H. Abdul Ridha Al-Awade2, H. Saeed Al-Mafragy3, N.N.H. AL Masaoodi4
1- General Directorate of Education in Karbala, Karbala, Iraq , batoolshakir5@gmail.com
2- Department of Biology, College of Education for Pure Sciences, Kerbala University, Kerbala, Iraq
3- College of Nursing, University of Warith Al-Anbiyaa, Karbala, Iraq
4- College of Medical and Health Technologies, Al-Zahraa University for Women, Karbala, Iraq
Abstract:   (461 Views)
Aims: Klebsiella pneumoniae can be defined as one of the virulent pathogens with a high level of antibiotic resistance. The goal of the presented work was to see if Capsicum annuum L. juice has antibacterial activity against K. pneumonia, a bacteria seen in respiratory tract infections.
Materials & Methods: Fifty respiratory tract infections have been collected that include such bacterium’s resistance to some antibiotics, the qualitative detection of C. annuum L. juice effective groups, the minimal inhibitory concentration of C. annuum L. juice, and the inhibition activity against the growth of K. pneumoniae, and its cytotoxicity was studied.
Findings: The cultural results showed that 12 (24%) isolates were related to K. pneumonia, and the associated virulence factors with K. pneumonia pathogenicity are capsule, hemolysin, urease, biofilm production, and β-lactamase production. All isolates showed resistance to 100% Ampicillin and indicated that juice contained high percentages of active compounds. The minimal inhibitory concentration value of C. annuum L. was 6.25mg/mL for the bacterial isolates related to K. pneumoniae. There has been a considerable difference at p≤0.050, while the cytotoxicity shows that Juice C. annuum L. has no cytotoxic activity against human red blood cells.
Conclusion: Antibacterial activity of C. annuum L. juice increases with the increase of its concentration, and it is possible to use it as an adjunct treatment to eliminate or reduce the growth of some pathogens.

Keywords: Anti-Bacterial Agents [MeSH], Klebsiella pneumoniae beta-lactamase CTX-M-2 [MeSH], Respiratory Tract Infections [MeSH]
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Article Type: Original Research | Subject: Veterans or Handicapped Health
Received: 2022/03/9 | Accepted: 2022/04/12 | Published: 2022/04/30
* Corresponding Author Address: General Directorate of Education in Karbala, Karbala, Iraq
1. Rock C, Thom KA, Masnick M, Johnson JK, Harris AD, Morgan DJ. Frequency of Klebsiella pneumoniae Carbapenemase (KPC)-producing and Non-KPC-producing klebsiella species contamination of healthcare workers and the environment. Infect Control Hosp Epidemiol. 2014;35(4):426-9. [Link] [DOI:10.1086/675598]
2. Anand T, Virmani N, Kumar S, Kumar Mohanty A, Pavulraj S, Bera BCh, et al. Phage therapy for treatment of virulent Klebsiella pneumoniae infection in a mouse model. J Glob Antimicrob Resist. 2020;21:34-41. [Link] [DOI:10.1016/j.jgar.2019.09.018]
3. Sidjabat H, Nimmo GR, Walsh TR, Binotto E, Htin A, Hayashi Y, et al. Carbapenem resistance in Klebsiella pneumoniae due to the New Delhi metallo-β-lactamase. Clin Infect Dis. 2011;52(4):481-4. [Link] [DOI:10.1093/cid/ciq178]
4. Podschun R, Ullmann U. Klebsiella spp. as nosocomial pathogens: epidemiology, taxonomy, typing methods, and pathogenicity factors. Clin Microbiol Rev. 1998;11(4):589-603. [Link] [DOI:10.1128/CMR.11.4.589]
5. Kumar V, Sun P, Vamathevan J, Li Y, Ingraham K, Palmer L, et al. Comparative genomics of Klebsiella pneumoniae strains with different antibiotic resistance profiles. Antimicrob Agents Chemother. 2011;55(9):4267-76. [Link] [DOI:10.1128/AAC.00052-11]
6. Navon-Venezia S, Kondratyeva K, Carattoli A. Klebsiella pneumoniae: a major worldwide source and shuttle for antibiotic resistance. FEMS Microbiol Rev. 2017;41(3):252-75. [Link] [DOI:10.1093/femsre/fux013]
7. Hou XH, Song XY, Ma XB, Zhang SY, Zhang JQ. Molecular characterization of multidrug-resistant Klebsiella pneumoniae isolates. Braz J Microbiol. 2015;46(3):759-68. [Link] [DOI:10.1590/S1517-838246320140138]
8. Damian M, Usein CR, Palade AM, Ceciu S, Cosman M. Molecular epidemiology and virulence characteristics of Klebsiella pneumoniae strains isolated from hospital-associated infections. Open Epidemiol J. 2009;2:69-78 [Link] [DOI:10.2174/1874297100902010069]
9. Brisse S, Fevre C, Passet V, Issenhuth-Jeanjean S, Tournebize R, Diancourt L, et al. Virulent clones of Klebsiella pneumoniae: identification and evolutionary scenario based on genomic and phenotypic characterization. PloS One. 2009;4(3):1-7. [Link] [DOI:10.1371/journal.pone.0004982]
10. Vuotto C, Longo F, Balice MP, Donelli G, Varaldo PE. Antibiotic resistance related to biofilm formation in Klebsiella pneumoniae. Pathogens. 2014;3(3):743-58. [Link] [DOI:10.3390/pathogens3030743]
11. Liaw SJ, Lai HC, Ho SW, Luh KT, Wang WB. Inhibition of virulence factor expression and swarming differentiation in Proteus mirabilis by p-nitrophenylglycerol. J Med Microbiol. 2000;49(8):725-31. [Link] [DOI:10.1099/0022-1317-49-8-725]
12. Ahmad VU, Hussain J, Hussain H, Jassbi AR, Ullah F, Lodhi MA, et al. First natural urease inhibitor from Euphorbia decipiens. Chem Pharmaceutic Bull. 2003;51(6):719-23. [Link] [DOI:10.1248/cpb.51.719]
13. Kayaoglu G, Ørstavik D. Virulence factors of Enterococcus faecalis: relationship to endodontic disease. Crit Rev Oral Biol Med. 2004;15(5):308-20. [Link] [DOI:10.1177/154411130401500506]
14. Riley MA, Chavan MA. Bacteriocins ecology and evolution. London: Springer-Verlag; 2007. [Link] [DOI:10.1007/978-3-540-36604-1]
15. Sarika AR, Lipton AP,Aishwarya MS. Bacteriocin production by a new isolate of Lactobacillus rhamnosus GP1 under different culture conditions. Adv J Food Sci Technol. 2010;2(5):291-7. [Link]
16. Al-Charrakh AH, Yousif SY, Al-Janabi HS. Antimicrobial spectrum of the action of bacteriocins from Klebsiella isolates from Hilla /Iraq. Iran J Microbiol. 2011;2(5):76-8. [Link]
17. Das A, Zaman K, Singh AV. Antimicrobial and antioxidant activities of Callistemon linearis DC leaf extract. Pharmacology. 2008;875-81. [Link]
18. Parekh J, Chanda S. In vitro antifungal activity of methanol extracts of some Indian medicinal plants against pathogenic yeast and moulds. Afr J Biotechnol. 2008;7(23):4349-53. [Link]
19. Vaidya AB, Antarkar VD. New drugs from medicinal plants: opportunities and approaches. J Assoc Physicians India. 1994;42(3):221-8. [Link]
20. Santosh K. Genetic variability studies in bell pepper (Capsicum annuum L.). Asian J Horticulture. 2013;8(1):280-4. [Link]
21. Shaha RK, Rahman S, Asrul A. Bioactive compounds in chilli peppers (Capsicum annuum L.) at various ripening (green, yellow and red) stages. Ann Biol Res. 2013;4(8):27-34. [Link]
22. Costa FR, Ferreira TNS, Vitoria AP, Campos KP, Rodrigues R, Silva DH, et al. Genetic diversity among Capsicum accessions using RAPD markers. Crop Breed Appl Biotechnol. 2006;6:18-23. [Link] [DOI:10.12702/1984-7033.v06n01a03]
23. John GH. Bergey's manual of determinative biology. 9th edition. Unknown city: William and Wilkins; 1994. pp. 181-6. [Link]
24. Atlas RM, Parks LC, Brown AE. Laboratory manual experimental microbiology. Unknown city: Mosby Yearbook, Inc;1995. p. 888. [Link]
25. Freeman DJ, Falkiner FR, Keane CT. New method for detecting slime production by coagulase negative staphylococci. J Clin Pathol. 1989;42(8):872-4. [Link] [DOI:10.1136/jcp.42.8.872]
26. Mathur T, Singhal S, Khan S, Upadhyay DJ, Fatma T, Rattan A. Detection of biofilm formation among the clinical isolates of staphylococci: an evaluation of three different screening methods. Indian J Med Microbiol. 2006;24(1):25-9. [Link] [DOI:10.1016/S0255-0857(21)02466-X]
27. Llanes R, González M, Martínez I, Sosa J, Guzmán D, Gutiérrez O, et al. Evaluation of four methods for detecting the beta-lactamase activity in Neisseria gonorrhoeae isolated in Cuba. Memórias do Instituto Oswaldo Cruz. 2003;98:1089-91. [Link] [DOI:10.1590/S0074-02762003000800020]
28. World Health Organization. Basic laboratory procedures in clinical bacteriology. Geneva: World Health Organization; 2003. [Link]
29. Anesini C, Perez C. Screening of plants used in argentine folk medicine for antimicrobial activity. J Ethnopharmacol. 1993;39(2):119-28. [Link] [DOI:10.1016/0378-8741(93)90027-3]
30. Kissinger PT. A textbook of pharmaceutical analysis, 3rd Ed. By KENNETH A. CONNORS Wiley-Interscience, 605 Third Avenue, New York, NY 10016. 1982. 664 pp. 15 × 23 cm. Price $55.00. 1984;73(7):1015. [Link] [DOI:10.1002/jps.2600730746]
31. Collee JG, Miles RS, Watt B. Tests for identification of bacteria. Mackie McCartney Pract Med Microbiol. 1996;14:131-49. [Link]
32. Soberon JR, Sgariglia MA, Sampietro DA, Quiroga EN, Vattuone MA. Antibacterial activity of plant extracts from northwestern Argentina. J Appl Microbiol. 2007;102(6):1450-61. [Link] [DOI:10.1111/j.1365-2672.2006.03229.x]
33. Perez C, Paul M, Bazerque P. Antibiotic assay by agar well diffusion method. Acta Biol Med Exp. 1990;15:113-5. [Link]
34. Xian-Guo H, Urasella M. Antifungal compound from Solanum nigrum. J Ethnopharm. 1994;43:173-7. [Link] [DOI:10.1016/0378-8741(94)90039-6]
35. Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing. Unknown city: Clinical and Laboratory Standards Institute; 2014. [Link]
36. Seltman HJ. Experimental design and analysis. Unknown city: STAT; 2012. [Link]
37. Nadasy KA, Domiati-Saad R, Tribble MA. Invasive Klebsiella pneumoniae syndrome in North America. Clinl Infect Dis. 2007;45(3):e25-8. [Link] [DOI:10.1086/519424]
38. Wanjala W, Nduko J, Mwende M. Coliforms contamination and hygienic status of milk chain in emerging economies . J Food Qual Hazards Control. 2018;5(1):3-10 [Link] [DOI:10.29252/jfqhc.5.1.3]
39. Almjalawi BSA, AL-Hamil ARH, Chechan RA. Antibacterial activity of aqueous extract Cinnamomum zeylanicun against of Klebsiella pneumoniae isolated from Bacteremic patients. AL-Qadisiyah Med J. 2019;15(2):150-7. [Link]
40. Almjalawi BSA, Al-Awade HARK, Al-Hamil ARH. Study the inhibition activity of Bifidobacterium spp. filtrate against some pathogenic bacteria isolated from patients with cardiac catheterization in-vitro. Int J Res Dev Pharm Life Sci. 2020;5:2099-106.‏ [Link]
41. Kumar A, Talwar A, Dhatwalia VK. Antimicrobial resistance patterns of klebsiella spp. isolated from raw milk of Doon valley. J Biotechnol. 2010(150):425-6. [Link] [DOI:10.1016/j.jbiotec.2010.09.590]
42. Wu MF, Yang CY, Lin TL, Wang JT, Yang FL, Wu SH, et al. Humoral immunity against capsule polysaccharide protects the host from magA+ Klebsiella pneumoniae-induced lethal disease by evading Toll-like receptor 4 signaling. Infect Immun. 2009;77(2):615-21. [Link] [DOI:10.1128/IAI.00931-08]
43. Almjalawi BSA, Alhamed TA, Almutteari AA. Antibacterial activity of biosurfactant extracted from Streptococcus thermophilus and its effect on some biochemical parameters in male rats. Caspian J Environ Sci. 2022;20(1):131-6. [Link]
44. de Freitas Ferreira J, Vieira EA, Nitschke M. The antibacterial activity of rhamnolipid biosurfactant is pH dependent. Food ResInt. 2019;116:737-44. [Link] [DOI:10.1016/j.foodres.2018.09.005]
45. Kuş H, Arslan U, Türk HD, Fındık D. Investigation of various virulence factors of Klebsiella pneumoniae strains isolated from nosocomial infections. Mikrobiyoloji bulteni. 2017;51(4):329-39. [Turkish] [Link] [DOI:10.5578/mb.59716]
46. Han JS, Jang IY, Ryu HS, Kim WY. Hemolysin gene expression in the hns Knockout mutant of Klebsiella pneumoniae UN strain. J Kor Soc Appl Biol Chem. 2010;53(4):401-6. [Link] [DOI:10.3839/jksabc.2010.062]
47. Carter EL, Flugga N, Boer JL, Mulrooney SB, Hausinger RP. Interplay of metal ions and urease. Metallomics. 2009;1(3):207-21. [Link] [DOI:10.1039/b903311d]
48. Montanaro L, Arciola CR, Baldassarri L, Borsetti E. Presence and expression of collagen adhesin gene (cna) and slime production in Staphylococcus aureus strains from orthopaedic prosthesis infections. Biomaterials. 1999;20(20):1945-9. [Link] [DOI:10.1016/S0142-9612(99)00099-X]
49. Götz F. Staphylococcus and biofilms. Mol Microbiol. 2002;43(6):1367-78. [Link] [DOI:10.1046/j.1365-2958.2002.02827.x]
50. Bordi C, de Bentzmann S. Hacking into bacterial biofilms: a new therapeutic challenge. Ann Intensive Care. 2011;1(1):19. [Link] [DOI:10.1186/2110-5820-1-19]
51. Hassan A, Usman J, Kaleem F, Omair M, Khalid A, Iqbal M. Evaluation of different detection methods of biofilm formation in the clinical isolates. Braz J Infect Dis. 2011;15(4):305-11. [Link] [DOI:10.1016/S1413-8670(11)70197-0]
52. de Jesus MB, Ehlers MM, Dos Santos RF, Kock MM. Review - Understanding β-lactamase Producing Klebsiella pneumoniae. Antimicrob Resistance Open Challenge. 2015;26:51. [Link] [DOI:10.5772/61852]
53. Benenson S, Temper V, Cohen MJ, Schwartz C, Hidalgo-Grass C, Block C. Imipenem disc for detection of KPC carbapenemase-producing Enterobacteriaceae in clinical practice. J Clin Microbiol. 2011;49(4):1617-20. [Link] [DOI:10.1128/JCM.02179-10]
54. Ghafourian S, Bin Sekawi Z, Sadeghifard N, Mohebi R, Neela VK, Maleki A, et al. The prevalence of ESBLs producing Klebsiella pneumoniae isolates in some major hospitals, Iran. Open Microbiol J. 2011;5:91-5. [Link] [DOI:10.2174/1874285801105010091]
55. Jiang MJ, Zhao SP, Li JM. Resistance of β-lactamase-producing Klebsiella pneumoniae to Imipenem with Ompk36 loss. Afr J Microbiol Res. 2012;6(6):1312-7. [Link] [DOI:10.5897/AJMR11.1603]
56. Al-Charrakh, Ala H, Allush, Murooj S, Al-Husayni, Widad K. Antibiotic susceptibility of enterobacteria isolated from one hospital in Hilla, Iraq. J Appl Pure Sci. 2011;19(1):36-45. [Arabic] [Link]
57. Sarojamma V, Ramakrishna V. Prevalence of ESBL-producing Klebsiella pneumoniae isolates in tertiary care hospital. Int Sch Res Notices. 2011;2011:318348. [Link] [DOI:10.5402/2011/318348]
58. Cowan MM. Plant products as antimicrobial agents. Clin Microbiol Rev. 1999;12(4):564-82. [Link] [DOI:10.1128/CMR.12.4.564]
59. Weiss RF, Fintelmann V. Herbal medicine. 2nd Edition. New York: Thieme; 2000. p. 438. [Link]
60. Laurence DR, Bennett PN, Brown MJ. Clinical pharmacology. 8th Edition. Edinburg: Churchill Livingstone; 1997. [Link]
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Shakir Abed Almjalawi B, Abdul Ridha Al-Awade H, Saeed Al-Mafragy H, AL Masaoodi N. Antibacterial Activity of Capsicum annum L. Juice Against Klebsiella pneumonia Isolated from Respiratory Tract Infections. Iran J War Public Health 2022; 14 (2) :139-146
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