Iranian Journal of War and Public Health

eISSN (English): 2980-969X
eISSN (Persian): 2008-2630
pISSN (Persian): 2008-2622
Volume 14, Issue 1 (2022)                   Iran J War Public Health 2022, 14(1): 105-109 | Back to browse issues page

Print XML PDF HTML Full-Text (HTML)


How to cite this article
Salman Z, Ghudhaib K. Association of Osteopontin and Alkaline Phosphatase in Male Patients with Diabetes Mellitus Type 2 and Periodontitis. Iran J War Public Health 2022; 14 (1) :105-109
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Rights and permissions
1- Department of Chemistry, College of Science for Women, University of Baghdad, Baghdad, Iraq
* Corresponding Author Address: Department of Chemistry, College of Science for Women, University of Baghdad, Iraq (
Abstract   (1076 Views)
Aims: Periodontitis considers one of the essential complications that are accompanied by diabetes mellitus disease. Subsequently, researchers are focusing on the early detection for the development of this disorder by employing suitable biochemical factors as an optimal marker. Accordingly, the current study aimed to assess the levels of both osteopontin and alkaline phosphatase in patients Sera who suffering from diabetes with periodontitis to knowledge their ability to be the best factors for early prediction.
Material & Methods: Blood samples were collected from 120 male persons, the age range of 35-65 years, in the Al-Baladiyat specialist center for dentistry. Patients were divided into three groups (30 with diabetic mellitus type 2, 30 with periodontitis, 30 patients with diabetic Mellitus with periodontitis) and 30 apparently healthy subjects as a management group. Serum levels of Fasting Blood Sugar and Alkaline phosphatase were estimated enzymatic method. Osteopontin levels were measured by the ELISA method.
Findings: The results showed an increase in osteopontin levels in patients groups of diabetes mellitus and diabetes mellitus with periodontitis compared to patients with only periodontitis and control groups. Also, results revealed a highly significant increase in Alkaline phosphatase levels in two patient groups including diabetic and diabetic with periodontitis in comparison with periodontitis and healthy groups. Receiver operating characteristic data analysis showed high values of the studied parameters in terms of AUC.
Conclusion: Analysis data by Receiver operating characteristic curve confirmed that both Osteopontin and Alkaline phosphatase consider excellent markers for the diagnosis of the studied cases based on the values of AUC. Accordingly, osteopontin was found to be the best marker that can be employed in the diagnosis of the studied cases

1. Hamid GS, Ghudhaib KK, Allawi AA. Association of type IV collagen with progression of diabetic nephropathy disease in Iraqi patients under typical criteria. Biochem Cell Arch. 2021;21(1):1471-5. [Link]
2. Hamid GS, Allawi AA, Ghudhaib KK. Correlation of pentosidine with kidney diseases in Iraqi patients with diabetic nephropathy. Iraqi J Sci. 2021:3436-42. [Link] [DOI:10.24996/ijs.2021.62.10.2]
3. Ghudhaib KK. Effect of alcoholic Catechin extract on hyperglycemia, hyperlipidemia and liver functions in Alloxan diabetic mice. Baghdad Sci J. 2014;11(3). [Link] [DOI:10.21123/bsj.11.3.1192-1200]
4. Ghudhaib KK, Turaki KM, Muzal SA. Estimation of serum Osteocalcin levels in osteoporotic postmenopausal Iraqi women with type 2 diabetes mellitus. Baghdad Sci J. 2014;11(4). [Link] [DOI:10.21123/bsj.11.4.1549-1555]
5. Ghudhaib KK, Ibrahim LM, Salman EA, Salman ZA. Effect of Zno nanoparticles on salivary peroxidase activity in chronic periodontitis patients (in vitro study). IOSR J Appl Chem. 2016;9(4). [Link]
6. Ibrahim L, Ghudhaib KK, Al-Rubaee EA, Salman ZA. Estimation of ZnO nanoparticles effect on salivary alp activity in chronic periodontitis patients: in vitro study. Int J Adv Res Biol Sci. 2016;3(4):152-9. [Link]
7. Ghudhaib KK, Ibrahim L, Al-Rubaee EA, Salman ZA. Assessment of ZnO nanoparticles effect on AST activity in Saliva of patients with chronic periodontitis: in vitro study. Int J Adv Res Biol Sci. 2016;3(6):179-86. [Link]
8. Ghudhaib KK, Ibrahim LM, Al-Rubaee EA, Salman ZA. Determination of Vmax and Km of ALP, AST and Peroxidase in Saliva of Chronic Periodontitis Patients with and without ZnO NPs: A Kinetic Study. Int J Adv Res Biol Sci. 2016;3(7):85-91. [Link]
9. Wu CZ, Yuan YH, Liu HH, Li SS, Zhang BW, Chen W, et al. Epidemiologic relationship between periodontitis and type 2 diabetes mellitus. BMC Oral Health. 2020;20:204. [Link] [DOI:10.1186/s12903-020-01180-w]
10. Salman ZA, Mezil SA, ALI VS, Kadim NM. Assessment of ZnO nanoparticles effect on peroxidase activity in serum of patients with type2 diabetes millets: in vitro study. Int J Pharmaceutical Res. 2019;11(2):76-80. [Link] [DOI:10.31838/ijpr/2019.11.02.014]
11. Fadhil R, Abdulameer LA, Salman ZA, Raheem ZJ. Effect of silicon dioxide nanoparticles in detection of serum peroxidase activity in severe periodontitis patients. Biochem Cell Arch. 2020;20(2):4971-5. [Link]
12. Panezai J, Altamash M, Engstrӧm PE, Larsson A. Association of Glycated proteins with inflammatory proteins and periodontal disease parameters. J Diabetes Res. 2020;2020:6450742. [Link] [DOI:10.1155/2020/6450742]
13. Koppolu P, Sirisha S, Mishra A, Deshpande K, Lingam AS, Alotaibi DH, et al. Alkaline phosphatase and acid phosphatase levels in saliva and serum of patients with healthy periodontium, gingivitis, and periodontitis before and after scaling with root planing: A clinico-biochemical study. Saudi J Biol Sci. 2021;28(1):380-5. [Link] [DOI:10.1016/j.sjbs.2020.10.016]
14. Salman ZA, Fadhil R, Kadim NM, Al-Rubaee EA. Effect of ZnO Nanoparticles on AST activity in gingival cervicular fluid of smokers and nonsmokers chronic periodontitis patients: in vitro study. J Glob Pharm Technol. 2018;10(3):375-80. [Link]
15. De A, Puttannavar R, Rahman F, Adak A, Sahoo R, Prakash BR. Estimation of salivary and serum alkaline phosphatase level as a diagnostic marker in type-2 diabetes mellitus with periodontal health and disease: a clinico-biochemical study. J Oral Maxillofac Pathol. 2018;22(3):445. [Link] [DOI:10.4103/jomfp.JOMFP_212_18]
16. Assy MH, El Ashmawy HM, Soliman JS, Abd El Hamed AB. Osteopontin as a marker of diabetic nephropathy in patients with type 2 diabetes mellitus. Egypt J Hospital Med. 2020;81(7):2439-44. [Link] [DOI:10.21608/ejhm.2020.133956]
17. Zhang Y, Zhou C, Li J, Zhang Y, Xie D, Liang M, Wang B, et al. Serum alkaline phosphatase levels and the risk of new-onset diabetes in hypertensive adults. Cardiovasc Diabetol. 2020;19(1):186. [Link] [DOI:10.1186/s12933-020-01161-x]
18. Salman ZA, Azeez RA, Yaseen AK, AL-Rubaee EA. Effect of zinc oxide nanoparticles on serum alkaline phosphatase activity in male mice. Biochem Cell Arch. 2018;18(2):1715-9. [Link]
19. Dennis M, Lee AR, McCarthy T. Nutritional considerations of the gluten-free diet. Gastroenterol Clin North Am. 2019;48(1):53-72 [Link] [DOI:10.1016/j.gtc.2018.09.002]
20. Kim K, Park SM. Association of muscle mass and fat mass with insulin resistance and the prevalence of metabolic syndrome in Korean adults: a cross-sectional study. Sci Rep. 2018;8(1):2703. [Link] [DOI:10.1038/s41598-018-21168-5]
21. Ghaib ZJ, Ghudhaib KK, Mohsen FY. Assessment of neuron specific enolase level and some related biochemical factors in patients with diabetic peripheral nerve disorders. Indian J Forensic Med Toxicol. 2021;15(3). [Link]
22. Mahmood IH, Abed MN, Merkhan MM. Effects of blocking of angiotensin system on the prevalence of metabolic syndrome in type 2 diabetic patients. Pak J Med Sci. 2013;29(1):140-3. [Link] [DOI:10.12669/pjms.291.2782]
23. Hassan KM, Balaji R, Gnaneswaran S, Kumar JS. To Study the association of HbA1C with retinopathy, neuropathy and high risk foot among diabetic patients attending rural tertiary care Hospital of Tamil Nadu, India. Int J Ophthalmol Eye Res. 2016;4(4):206-11. [Link] [DOI:10.19070/2332-290X-1600042]
24. Pan HZ, Zhang L, Guo MY, Sui H, Li H, Wu WH, et al. The oxidative stress status in diabetes mellitus and diabetic nephropathy. Acta Diabetol. 2010; 47(Suppl 1):71-6. [Link] [DOI:10.1007/s00592-009-0128-1]
25. Preshaw PM, Bissett SM. Periodontitis and diabetes. Br Dent J. 2019;227(7):577-84. [Link] [DOI:10.1038/s41415-019-0794-5]
26. Khanuja PK, Narula SC, Rajput R, Sharma RK, Tewari S. Association of periodontal disease with glycemic control in patients with type 2 diabetes in Indian population. Front Med. 2017;11(1):110-9. [Link] [DOI:10.1007/s11684-016-0484-5]
27. Jeftha A, Roberts T, Kimmie-Dhansay F. The effect of periodontal disease on metabolic control in patients with diabetes mellitus in South Africa: Protocol for a Systematic Review. JMIR Res Protoc. 2021;10(7):e27471. [Link] [DOI:10.2196/27471]
28. Emrich LJ, Shlossman M, Genco RJ. Periodontal disease in non‐insulin‐dependent diabetes mellitus. J Periodontol. 1991;62(2):123-31. [Link] [DOI:10.1902/jop.1991.62.2.123]
29. Mochida Y, Duarte WR, Tanzawa H, Paschalis EP, Yamauchi M. Decorin modulates matrix mineralization in vitro. Biochem Biophys Res Commun. 2003;305(1):6-9. [Link] [DOI:10.1016/S0006-291X(03)00693-4]
30. Nakamura M, Slots J. Salivary enzymes: origin and relationship to periodontal disease. J Periodontal Res. 1983;18(6):559-69. [Link] [DOI:10.1111/j.1600-0765.1983.tb00393.x]
31. Chen J, Singh K, Mukherjee BB, Sodek J. Developmental expression of osteopontin (OPN) mRNA in rat tissues: evidence for a role for OPN in bone formation and resorption. Matrix. 1993;13(2):113-23. [Link] [DOI:10.1016/S0934-8832(11)80070-3]
32. Yan X, Sano M, Lu L, Wang W, Zhang Q, Zhang R, et al. Plasma concentrations of osteopontin, but not thrombin-cleaved osteopontin, are associated with the presence and severity of nephropathy and coronary artery disease in patients with type 2 diabetes mellitus. Cardiovasc Diabetol. 2010;9:70. [Link] [DOI:10.1186/1475-2840-9-70]

Add your comments about this article : Your username or Email: