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Iran J War Public Health 2022, 14(1): 111-118 Back to browse issues page
Immune Responses in Patient Infected with Entamoeba histolytica and the Antigensity of Cyst in Basrah Province, Iraq
S.A. Nassar1, S.R. Al-Idreesi *2, Gh.Y. Azzal1
1- Department of Microbiology, College of Veterinary Medicine, Basrah University, Basrah, Iraq
2- Department of Microbiology, College of Veterinary Medicine, Basrah University, Basrah, Iraq , suhair.riyadh@gmail.com
Abstract:   (110 Views)
Aims: Amoebiasis was a main parasitic infestation triggered via Entamoeba histolyticawith clinical signs assortments starting moderately to heavy. This study aimed to culture E. histolytica in vitro from the stool samples of humans, and the stage of the cyst was purified.
Materials & Methods: Stool plus blood samples were collected from patients attending Basrah Hospitals. The identification of E. histolytica was done by macroscopic and microscopical examination. The high efficiency was recorded in the culture medium for isolating E. histolytica from the stool samples of humans. The cysts were purified.
Findings: The purity by use of precoll gradient is higher than in the culture medium and the protein of E. histolytica cysts was prepared. The immunogenicity of E. histolytica cysts was considered by using SDS-PAGE and Western blot. Seven proteins had been valued in patient serum as more immunogenic, their molecular weight was (83, 69, 61, 34, 29.1, 18.1, and 11.2kDa). Also, the intensities of IL-4 and IFN-γ were assessed by using ELISA kits in the serum of amoebiasis patients.
Conclusion: This study revealed that the serum level of IL-4 was significantly higher in patients with E. histolytica associated with healthy observation collection, while IFN-γ showed no significant difference.
 
Keywords: Antigens [MeSH], Cysts [MeSH], Entamoeba histolytica [MeSH], Western Blot [MeSH],
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Article Type: Original Research | Subject: Epidemiology and Statistics of War/Accident Related Injuries
Received: 2022/03/11 | Accepted: 2022/04/12 | Published: 2022/01/20
* Corresponding Author Address: Department of Microbiology, College of Veterinary Medicine, Basrah University, Aljazyr Street, Basrah, Iraq, Postal Code: 42001
References
1. Fletcher SM, Stark D, Harkness J, Ellis J. Enteric protozoa in the developed world: A public health perspective. Clin Microbiol Rev. 2012;25(3):420-49. [] [DOI:10.1128/CMR.05038-11] [PMID] [PMCID]
2. Kelly P. Protozoal gastrointestinal infections. Medicine. 2013;41(12):705-8. [] [DOI:10.1016/j.mpmed.2013.09.003]
3. Monzote L, Siddiq A. Drug development to protozoan diseases. Open Med Chem J. 2011;5:1-3. [] [DOI:10.2174/1874104501105010001] [PMID] [PMCID]
4. Lebbad M, Svard SG. PCR differentiation of Entamoeba histolytica and Entamoeba dispar from patients with amoeba infection initially diagnosed by microscopy. Scand J Infect Dis. 2005;37(9):680-5. [] [DOI:10.1080/00365540510037812] [PMID]
5. Ohnishi K, Kato Y, Imamura A, Fukayama M, Tsunoda T, Sakaue Y, et al. Present characteristics of symptomatic Entamoeba histolytica infection in the big cities of Japan. Epidemiol Infect. 2004;132(1):57-60. [] [DOI:10.1017/S0950268803001389] [PMID] [PMCID]
6. Xim'enez C, Mor'an P, Rojas L, Valadez A, Gomez A. "Reassessment of the epidemiology of amebiasis: State of the art," Infection, Genetics and Evolution, 2009;9(6):1023-32. [] [DOI:10.1016/j.meegid.2009.06.008] [PMID]
7. Voigt H, Olivo JC, Sansonetti P, Guillén N. Myosin IB from Entamoeba histolytica is involved in phagocytosis of human erythrocytes. J Cell Sci. 1999; 112 (Pt 8):1191-201. [] [DOI:10.1242/jcs.112.8.1191] [PMID]
8. Wilson IW, Weedall GD, Hall N. Host-parasite interactions in Entamoeba histolytica and Entamoeba dispar: What have we learned from their genomes?. Parasite Immunol. 2012;34(2-3):90-9. [] [DOI:10.1111/j.1365-3024.2011.01325.x] [PMID] [PMCID]
9. Yu Y, Chadee K. Entamoeba histolytica stimulates interleukin 8 from human colonic epithelial cells without parasite-enterocyte contact. Gastroenterology. 1997;112(5):1536-47. [] [DOI:10.1016/S0016-5085(97)70035-0]
10. Guerrant RL, Brush J, Ravdin JI, Sullivan JA, Mandell GL. Interaction between Entamoeba histolytica and human polymorphonuclear neutrophils. J Infect Dis. 1981;143:83-93. [] [DOI:10.1093/infdis/143.1.83] [PMID]
11. Denis M, Chadee K. Human neutrophils activated by interferongamma and tumour necrosis factor-alpha kill Entamoeba histolytica trophozoites in vitro. J Leukoc Biol. 1989;46:270-274. [] [DOI:10.1002/jlb.46.3.270] [PMID]
12. Guo X, Barroso L, Lyerly DM, Petri Jr WA, Houpt ER. CD4+ and CD8+ T cell- and IL-17-mediated protection against Entamoeba histolytica induced by a recombinant vaccine. Vaccine. 2011;29(4):772-7. [] [DOI:10.1016/j.vaccine.2010.11.013] [PMID] [PMCID]
13. Gay NJ, Gangloff M. Structure and function of toll receptors and their ligands. Annu Rev Biochem. 2007;76:141-65. [] [DOI:10.1146/annurev.biochem.76.060305.151318] [PMID]
14. Galvan-Moroyoqui JM, Del CarmenDomínguez-Robles M, Meza I. Pathogenic bacteria prime the induction of toll-like receptor signalling in human colonic cells by the Gal/GalNAc lectin carbohydrate recognition domain of Entamoeba histolytica. Int J Parasitol. 2011;41(10):1101-12. [] [DOI:10.1016/j.ijpara.2011.06.003] [PMID]
15. Kumar H, Kawai T, Akira S. Pathogen recognition by the innate immune sys-tem. Int Rev Immunol. 2011;30:16-34. [] [DOI:10.3109/08830185.2010.529976] [PMID]
16. Sanchez-Guillen M. del. C, Perez-Fuentez R, Salgado-Rosas H, Ruiz-Arguelles A, Ackers J, Shire A, Talmas-Rohana P. Differentiation of Entamoeba histolytica /Entamoeba dispar by PCR and their correlation with humoral and cellular immunity in individuals with clinical variants of amoebiasis. Am J Trop Med Hyg. 2002;66(6):731-7. [] [DOI:10.4269/ajtmh.2002.66.731] [PMID]
17. Al-Shaheen Z, Al-Maki AK, Hussein K. A study on prevalence of Entamoeba histolytica & Giardia lamblia infection among patient attending Qurna hospital in Basrah. Basrah J Vet Res. 2007;6:30-6. [] [DOI:10.33762/bvetr.2007.55769]
18. Al-Idreesi SR, Al-Azizz S, Azzal Gh, Hisab A. Culture of Entamoeba histolytica in vitro. AL-Qadisiya J Vet Med Sci. 2008;7(1). []
19. Weber R, Bryan RT, Juranek DD. Improved stool concentration procedure for detection of Cryptosporidium oocysts in fecal specimens. J Clin Microbiol. 1992;30(11):2869-73. [] [DOI:10.1128/jcm.30.11.2869-2873.1992] [PMID] [PMCID]
20. Segovia-Gamboa NC, Chavez-Mongolia B, Medina-Flores Y, Cazares-Raga FE, Hernandez-Ramirez VI. Entamoeba invadens, encystation process and enolase. Exp Parasitol. 2010;125:63-9. [] [DOI:10.1016/j.exppara.2009.12.019] [PMID]
21. Nassar SA, Al-Idreesi SR, Al-Emaara GY. Epidemiological study of Entameoba histolytica in Basrah city -southern of Iraq. Bas J Vet Res. 2019;18(2).
22. Dagci H, Balcioglu IC, Ertabaklar H, Kurt O, Atambay M. Effectiveness of peptone-yeast extract (P-Y) medium in the cultivation and isolation of Entamoeba histolytica/Entamoeba dispar in Turkish patients. Diagn Microbiol Infect Dis. 2003;45(2):127-30. [] [DOI:10.1016/S0732-8893(02)00508-4]
23. Clark CG, Diamond LS. Methods for cultivation of luminal parasitic protists of clinical importance. Clin Microbiol Rev. 2002;15(3):329-41. [] [DOI:10.1128/CMR.15.3.329-341.2002] [PMID] [PMCID]
24. Eichinger D. A role for a galactose lectin and its ligands during encystment of Entamoeba. J Eukaryot Microbiol. 2001;48(1):17-21. [] [DOI:10.1111/j.1550-7408.2001.tb00411.x] [PMID]
25. Makioka A, Kumagai M, Ohtomo H, Kobayashi S, Takeuchi T. Effect of calcium antagonists, calcium channel blockers and calmodulin inhibitors on the growth and encystations of Entamoeba histolytica and E. invadens. Parasitol Res. 2001;87:833-7. [] [DOI:10.1007/s004360100453] [PMID]
26. Que X, Reed SL. Cysteine proteinases and the pathogenesis of amebiasis. J Clin Microbiol Rev. 2000;13(2):196-204. [] [DOI:10.1128/CMR.13.2.196] [PMID]
27. Garcia-Rivera G, Rodriguez MA, Ocadiz R, Martinez-Lopez MC, Arroyo R, Gonzalez-Robles A, Orozco E. Entamoeba histolytica: a novel cysteine protease and an adhesin form the 112 kDa surface protein. Mol Microbiol. 1999;33(3):556-68. [] [DOI:10.1046/j.1365-2958.1999.01500.x] [PMID]
28. Banuelos C, Garcia-Rivera G, Lopez-Reyes I, Mendoza L, Gonzalez-Robles A, Herranz S, et al. EhADH112 is a Bro1 domain-containing protein involved in the Entamoeba histolytica multivesicular bodies pathway. J Biomed Biotechnol. 2012;2012:657942. [] [DOI:10.1155/2012/657942] [PMID] [PMCID]
29. Wong WK, Tan ZN, Othman N, Lim BH, Mohamed Z, Olivos Garcia A, Noordin R. Analysis of Entamoeba histolytica excretory-secretory antigen and identification of a new potential diagnostic marker. Clin Vaccine Immunol. 2011;18(11):1913-7. [] [DOI:10.1128/CVI.05356-11] [PMID] [PMCID]
30. Min X, Feng M, Guan Y, Man S, Fu Y, Cheng X, et al. Evaluation of the C-terminal fragment of Entamoeba histolytica Gal/GalNAc lectin intermediate subunit as a vaccine candidate against amebic liver abscess. PLoS Negl Trop Dis. 2016;10:e0004419. [] [DOI:10.1371/journal.pntd.0004419] [PMID] [PMCID]
31. Mortimer L, Chadee K. The immunopathogenesis of Entamoeba histolytica. Exp Parasitol. 2010;126(3):366-80. [] [DOI:10.1016/j.exppara.2010.03.005] [PMID]
32. Pacheco-Yépez J, Rivera-Aguilar V, Barbosa-Cabrera E, Rojas Hernández S, Jarillo-Luna RA, Campos-Rodríguez R. Myeloperoxidase binds to and kills Entamoeba histolytica trophozoites. Parasite Immunol. 2011;33(5):255-64. [] [DOI:10.1111/j.1365-3024.2010.01275.x] [PMID]
33. Rafiei A, Ajami A, Hajilooi M, Etemadi A. Th-1/ Th-2 cytokine pattern in human amoebic colitis. Pak J Biol Sci. 2009;12(20):1376-80. [] [DOI:10.3923/pjbs.2009.1376.1380] [PMID]
34. Guo X, Barroso L, Becker SM, Lyerly DM, Vedvick TS, Reed SG, et al. Protection against intestinal amebiasis by a recombinant vaccine is transferable by T cells and mediated by gamma interferon. Infect Immun. 2009;77(9):3909-18. [] [DOI:10.1128/IAI.00487-09] [PMID] [PMCID]
35. Bernin H, Marggraff C, Jacobs T, Brattig N, van AN L, Blessmann J, et al. Immune markers characteristic for asymptomatically infected and diseased Entamoeba histolytica individuals and their relation to sex. BMC Infect Dis. 2014;14:621. [] [DOI:10.1186/s12879-014-0621-1] [PMID] [PMCID]
36. Moraes LC, Al França EL, Pessoa RS, Fagundes DLG, Hernandes MG, PRibeiro V, et al. The effect of IFN-γ and TGF-β in the functional activity of mononuclear cells in the presence of Entamoeba histolytica. Parasites Vect. 2015;8:413. [] [DOI:10.1186/s13071-015-1028-6] [PMID] [PMCID]
37. Gonzalez Rivas E, Ximenez C, Nieves-Ramirez ME, Moran Silva P, Partida-Rodríguez O, Hernandez EH, et al. Entamoeba histolytica calreticulin induces the expression of cytokines in peripheral blood mononuclear cells isolated from patients with amebic liver abscess. Front Cell Infect Microbiol. 2018;8:358. [] [DOI:10.3389/fcimb.2018.00358] [PMID] [PMCID]
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Nassar S, Al-Idreesi S, Azzal G. Immune Responses in Patient Infected with Entamoeba histolytica and the Antigensity of Cyst in Basrah Province, Iraq. Iran J War Public Health. 2022; 14 (1) :111-118
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Volume 14, Issue 1 (2022) Back to browse issues page
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