Iranian Journal of War and Public Health

eISSN (English): 2980-969X
eISSN (Persian): 2008-2630
pISSN (Persian): 2008-2622
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Volume 15, Issue 1 (2023)                   Iran J War Public Health 2023, 15(1): 101-105 | Back to browse issues page

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Banoon S, Hussein Ali Z, Al-Kraety I, Aziz Z. Molecular Detection of blaTEM and blaCTX-M Encoding Genes from Klebsiella oxytoca Isolates from Tonsillitis. Iran J War Public Health 2023; 15 (1) :101-105
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1- Department of Biology, College of Science, University of Misan, Misan, Iraq
2- Alameen Center for Advanced Research and Biotechnology, Imam Ali Holy Shrine, Najaf, Iraq
3- Department of Medical Laboratory Techniques, Faculty of Health and Medical Techniques, University of Alkafeel, Najaf, Iraq
* Corresponding Author Address: Department of Biology, College of Science, University of Misan, Misan, Iraq. Postal Code: 62001 (shimarb@uomisan.edu.iq)
Abstract   (592 Views)
Aims: Tonsillitis is inflammation of the tonsils, a common clinical state caused by bacterial or viral infections. There are different types of tonsillitis; acute, sub-acute, chronic, and recurrent. The aim of this study was the isolation and identification of Klebsiella oxytoca isolated from tonsillitis based on conventional standard bacteriological methods and confirmed by VITEK-2 compact system.
Materials & Methods: A polymerase chain reaction was performed to detect blaCTX-M and blaTEM genes.
Findings: A total of 50 specimens were recovered from tonsillitis using swab sampling, which contained 35 bacterial growths. Onto the MacConkey agar, 15 isolates were confirmed as K. oxytoca using IMVIC test and VITEK-2 compact system. In the genotypic test, K. oxytoca isolates contained 11 (73.3%) blaCTX-M and 10 (66.6%) blaTEM genes.
Conclusion: The use of the VITEK-2 system is necessary to confirm the precise identification of K. oxytoca nosocomial pathogens from tonsillitis. The existence of blaTEM and blaCTX-M gene in half of K. oxytoca isolates is a concern that needs control strategies.
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