Iranian Journal of War and Public Health
طب جانباز
Iran J War Public Health
Medical Sciences
http://ijwph.ir
153
ijwph
2008-2622
2008-2630
10.58209/ijwph
en
jalali
1401
4
1
gregorian
2022
7
1
14
3
online
1
fulltext
en
Frequency of Inducible Clindamycin Resistance in Staphylococcus haemolyticus Isolated from Surgical Wounds Infections Using D-test and Molecular Methods in Al-Basrah, Iraq
آمار و اپیدمیولوژی معلولیتهای ناشی از جنگ/حادثه
Epidemiology and Statistics of War/Accident Related Injuries
پژوهشی اصيل
Original Research
<span style="font-size:10pt"><span style="text-autospace:none"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span cambria="" lang="EN-GB" style="font-family:">Aims: </span></b><i><span cambria="" lang="EN-GB" style="font-family:">S. haemolyticus</span></i><span cambria="" lang="EN-GB" style="font-family:"> is generally considered an opportunistic pathogen that is strongly associated with immunocompromised individuals. <i>S. haemolyticus</i> is ranked as a highly antibiotic-resistant pathogen for various types of antibiotics. Current study aimed to investigate the frequency of inducible clindamycin resistance in <i>S. haemolyticus</i> isolated from surgical wounds infections using D-test and molecular methods in Al-Basrah, Iraq.</span><b><span lang="EN-GB" style="font-family:"Cambria",serif"></span></b></span></span></span></span><br>
<span style="font-size:10pt"><span style="text-autospace:none"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span cambria="" style="font-family:">Materials & Methods: </span></b><span cambria="" lang="EN-GB" style="font-family:">200 surgical wound swabs were collected from Ports General Hospital in Basrah, Iraq. The coagulase-negative staphylococcal strains were identified using methods like oxidase, catalase, hemolysis, and coagulase tests and confirmed by Vitek®2 system. Methicillin resistance and inducible clindamycin resistance were detected according to disk diffusion method based on CLSI guidelines. Moreover, molecular approaches was performed to confirm methicillin and inducible clindamycin resistance results.</span></span></span></span></span><br>
<span style="font-size:10pt"><span style="text-autospace:none"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span cambria="" lang="EN-GB" style="font-family:">Findings:</span></b><span cambria="" lang="EN-GB" style="font-family:"> Out of 200 cases, 75 surgical wound swabs (37.5%) showed positive bacterial cultures. The highest frequency of isolates belonged to <i>Pseudomonas aeruginosa</i> (25.3%), <i>Staphylococcus epidermidis </i>(17.3%), <i>Staphylococcus aureus</i> (14.7%) and <i>Escherichia coli </i>(13.3%), respectively. Out of eight <i>S. haemolyticus isolates</i>, only 5 isolates (62.5%) showed inhibitory resistance criteria for both oxacillin and cefoxitin. Furthermore, 3 <i>S. haemolyticus </i>isolates (37.5%) were erythromycin-resistant and clindamycin sensitive with D-test positive with iMLS<sub>B</sub> resistance phenotype. While 2 isolates (25.0%) showed cMLS<sub>B</sub> resistance phenotype and 3 (37.5%) isolates were shown MS<sub>B</sub> resistance phenotypes. The most frequent resistance genes of <i>S. haemolyticus</i> strains were <i>mecA</i> (62.5%), <i>ermA</i> (62.5%), <i>ermB</i> (50.0%), respectively.</span></span></span></span></span><br>
<span style="font-size:10pt"><span style="text-autospace:none"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span cambria="" lang="EN-GB" style="font-family:">Conclusion: </span></b><span cambria="" lang="EN-GB" style="font-family:">D-test and molecular technique are appropriate for detection of inducible clindamycin resistance in <i>S. haemolyticus</i> strains.</span><b><span lang="EN-GB" style="font-family:"Cambria",serif"></span></b></span></span></span></span><br>
<span style="font-size:10pt"><span style="text-autospace:none"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span style="font-size:9.5pt"><span cambria="" style="font-family:"><span style="color:red"></span></span></span></span></span></span></span>
Staphylococcus haemolyticus,Multiple Drug Resistance,Methicillin Resistance,Clindamycin,
351
357
http://ijwph.ir/browse.php?a_code=A-10-1997-1&slc_lang=en&sid=1
A.A.
Al-Mousawi
15300319475328460024086
15300319475328460024086
Yes
Department of Biology, College of Science, University of Basrah, Basrah, Iraq
S.S.
Al-Amara
15300319475328460024087
15300319475328460024087
No
Department of Biology, College of Science, University of Basrah, Basrah, Iraq
M.N.
Humoud
15300319475328460024088
15300319475328460024088
No
Department of Pathological Analysis, College of Science, University of Basrah, Basrah, Iraq
S.O.
Hattab
15300319475328460024089
15300319475328460024089
No
Public Health Department, Basrah Health Directorate, Basrah, Iraq