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Volume 15, Issue 1 (2023)
Iran J War Public Health 2023, 15(1): 43-48 |
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Received: 2022/06/29 | Accepted: 2023/01/30 | Published: 2023/03/1
Received: 2022/06/29 | Accepted: 2023/01/30 | Published: 2023/03/1
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Mezban J, Abbas B, Khudor M. Erythromycin Resistance Genes among Coagulase-negative Staphylococci Isolated from Humans in Basrah, Iraq. Iran J War Public Health 2023; 15 (1) :43-48
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1- Department of Veterinary Microbiology and Parasitology, College of Veterinary Medicine, University of Basrah, Iraq
Abstract (495 Views)
Aims: In recent years, the global incidence of infections caused by gram-negative bacteria resistant to antibiotics has increased. This study aimed to investigate the presence and frequency of coagulase-negative Staphylococci in contact between animals and people and determine the phenotypic antimicrobial resistance profiles of coagulase-negative Staphylococci isolates from these sources.
Materials & Methods: 80 samples were collected from humans in different areas of Basrah Province, including 40 samples from human hand swabs and 40 from nasal swabs. The samples were inoculated onto mannitol salt agar and blood agar and then incubated at 37ºC for 24 hrs. Antibiotic susceptibility testing was performed using the disc diffusion method. A molecular study was done using the PCR technique.
Findings: 37 samples (46.25%) were positive for staphylococcal infection. Five species, including S. sciuri, S. lentus, S. gallinarum, S. chromogen, and S. haemolyticus were identified, according to Vitek 2 kit. Staphylococci were resistant to several different antibiotics. Out of 20 amplification samples, only 12 positive samples were purified for the ermA gene region with a PCR product of 190 bp. The results also showed the presence of an ermC band with a size of 299 bp, which represents the correct expected band in 8 isolates out of all isolates.
Conclusion: Gram-positive organisms are increasingly identified as the source of acute clinical infection in animals and humans. Some isolates are resistant to several different antibiotics. The ermC gene, ermA gene, and both ermA and ermC genes are present in the genome of these bacteria.
Materials & Methods: 80 samples were collected from humans in different areas of Basrah Province, including 40 samples from human hand swabs and 40 from nasal swabs. The samples were inoculated onto mannitol salt agar and blood agar and then incubated at 37ºC for 24 hrs. Antibiotic susceptibility testing was performed using the disc diffusion method. A molecular study was done using the PCR technique.
Findings: 37 samples (46.25%) were positive for staphylococcal infection. Five species, including S. sciuri, S. lentus, S. gallinarum, S. chromogen, and S. haemolyticus were identified, according to Vitek 2 kit. Staphylococci were resistant to several different antibiotics. Out of 20 amplification samples, only 12 positive samples were purified for the ermA gene region with a PCR product of 190 bp. The results also showed the presence of an ermC band with a size of 299 bp, which represents the correct expected band in 8 isolates out of all isolates.
Conclusion: Gram-positive organisms are increasingly identified as the source of acute clinical infection in animals and humans. Some isolates are resistant to several different antibiotics. The ermC gene, ermA gene, and both ermA and ermC genes are present in the genome of these bacteria.
Keywords:
Erythromycin [MeSH], Antibiotic Resistance [MeSH], Bacterial Genes [MeSH], Staphylococcus [MeSH], Human [MeSH], PCR [MeSH]
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