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Molecular Detection of bla TEM and bla CTX-M encoding genes from Klebsiella oxytoca Isolates from Tonsillitis
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| Shaima Rabeea Banoon *1, Zahraa Hussein Ali2, Israa Abdul Ameer Al-Kraety3, Zahid S. Aziz4 |
1- Department of Biology, College of Science, University of Misan, Misan, Iraq , shimarb@uomisan.edu.iq
2- Alameen Center for Advanced Research and Biotechnology, Imam Ali Holy Shrine, Najaf, Iraq
3- Department of Medical Laboratory Techniques, Faculty of Health and Medical Techniques, University of Alkafeel, Najaf, Iraq
4- Department of Biology, College of Science, University of Misan, Misan, Iraq |
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Abstract: (134 Views) |
Tonsillitis is inflammation of the tonsils, a common clinical state caused by bacterial or viral infections. There are several types of tonsillitis including acute, sub-acute, chronic, and recurrent. Chronic tonsillitis is referred to enlargement of the tonsils along with recurrent infective attacks. It is the most common throat disease which is mainly observed in younger individuals. The aim of this study was the isolation and identification Klebsiella oxytoca (K. oxytoca) from tonsillitis based on diagnostic procedures. Polymerase chain reaction was performed to detect blaCTXM and blaTEM genes. A total of thirty-five specimens were recovered from tonsillitis using swab sampling, represented by 35 specimens which contained 30 bacterial growth. The 21 of bacteria were lactose fermentative onto the MacConkey agar. Twenty of 30 suspected isolates were confirmed as K. oxytoca using VITEK-2 compact system. In genotypic test, K. oxytoca isolates contained 11 (55%) blaCTXM 10 (50%) blaTEM being responsible for cephalosporines resistance.
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| Keywords: Klebsiella oxytoca, blaTEM, blaCTXM, Tonsillitis |
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Full-Text [PDF 931 kb]
(81 Downloads)
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Article Type: Original Research |
Subject:
Veterans or Handicapped Health
Received: 2022/11/26 | Accepted: 2023/03/8 | Published: 2023/03/16
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