Aims:
Sulfur mustard causes blister and was used for the first time as a chemical
weapon in World War I and during the Iran-Iraq war as well. The
aim of this study was to investigate the correlation of C→T polymorphism in
-509 promoter region of TGF-β1 gene with long-term pulmonary complications in sulfur mustard chemical injures.
Materials & Methods: This study was done on 252 chemically-injured
veterans including 107 exposed subjects with pulmonary problems and 145
subjects without pulmonary problems. Peripheral
blood samples were collected in the tubes containing EDTA. Then, genomic DNA was extracted of peripheral blood
leukocytes. PCR-RFLP method was used to determine genotype of the
subjects. Statistical analysis was done using
SPSS 18 by Chi-square test.
Findings: 33
subjects in chemically-injured patients with pulmonary problems group and 43
subjects in groups without pulmonary problems had CC homozygous genotype.
59 chemically-injured patients with pulmonary
problems and 76 subjects of the group without pulmonary problems had CT
heterozygous genotype (p=0.967). Also, 15 subjects
of pulmonary problems group and 26 subjects of the group without pulmonary problems
had TT homozygous genotype (p=0.473). No significant difference was
observed between different genotype distribution of the polymorphism of C→T in -509 promoter region of TGF-β1 gene of with and without
pulmonary problem groups.
Conclusions: C→T polymorphism in -509 promoter region of TGF-β1 gene is not correlated with pulmonary problems in chemical injured veterans.