JMERC
Owner
Janbazan Medical and Engineering Research Center (JMERC) is the owner of IJWPH.
0.3
Cite Score
SJR: 0.114 / SNIP: 0.090
Volume 13, Issue 4 (2021)
Iran J War Public Health 2021, 13(4): 305-311 |
Back to browse issues page
Article Type:
Subject:
History
Received: 2021/12/27 | Accepted: 2021/12/27 | Published: 2022/02/12
Received: 2021/12/27 | Accepted: 2021/12/27 | Published: 2022/02/12
How to cite this article
Dhahir Mansour Al Sultani T, Ayad Fakhri S, Al-Zuhairi W. Comparison of the Antimicrobial Activity of Nigella sativa Aqueous and Oil Extracts. Iran J War Public Health 2021; 13 (4) :305-311
URL: http://ijwph.ir/article-1-1093-en.html
URL: http://ijwph.ir/article-1-1093-en.html
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Rights and permissions
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
1- Department of Microbiology, Al-Esraa University College, Baghdad, Iraq
2- Department of Pharmacology and Toxicology, Al-Esraa University College, Baghdad, Iraq
3- Pharmacy Department, Al-Esraa University College, Baghdad, Iraq
2- Department of Pharmacology and Toxicology, Al-Esraa University College, Baghdad, Iraq
3- Pharmacy Department, Al-Esraa University College, Baghdad, Iraq
Abstract (1159 Views)
Aims: Nigella sativa is recognized as a black seed. It is a grassy plant relating to the Ranunculaceae family. There are various reports regarding this plant's pharmacological and biological action, like antihypertensive effects, antibacterial, anticancer, antioxidants, antifungal, pain alleviating, anti-inflammatory, antidiabetic, and immune-modulatory effects. This study aimed to compare the anti-microbial activity of aqueous and oil extract of Nigella sativa against selected gram-positive and gram-negative bacteria.
Materials & Methods: Nigella sativa aqueous and oil extracts were gathered via a retail food shop (Al-Hilla) 2018. Gram-positive (Staphylococcus aureus; Streptococcus pneumonia; Streptococcus pyogenes) and Gram-negative (Salmonella typhi; Escherichia coli; Pseudomonas aeroginosa) isolates (obtained via clinical specimens) were utilized.
Findings: Both Nigella sativa aqueous and oil extracts showed a maximum inhibition zone against E. coli and minimum inhibition against S. pyogenes.
Conclusion: Nigella sativa acts against gram-positive as well as gram-negative bacterial isolates.
Materials & Methods: Nigella sativa aqueous and oil extracts were gathered via a retail food shop (Al-Hilla) 2018. Gram-positive (Staphylococcus aureus; Streptococcus pneumonia; Streptococcus pyogenes) and Gram-negative (Salmonella typhi; Escherichia coli; Pseudomonas aeroginosa) isolates (obtained via clinical specimens) were utilized.
Findings: Both Nigella sativa aqueous and oil extracts showed a maximum inhibition zone against E. coli and minimum inhibition against S. pyogenes.
Conclusion: Nigella sativa acts against gram-positive as well as gram-negative bacterial isolates.
References
1. Halawani E. Antibacterial activity of Thymoquinone and Thymohydroquinone of Nigella sativa L. and their interaction with some antibiotics. Adv Biol Res. 2009;3(5-6):148-52. [Link]
2. Okeke MI, Iroegbu CU, Eze EN, Okoli AS, Esimone CO. Evaluation of extracts of the root of Landolphia owerrience for antibacterial activity. J Ethnopharmacol. 2001;78(2-3):119-27. [Link] [DOI:10.1016/S0378-8741(01)00307-5]
3. Najah AM. Effect of Nigella sativa L. extracts against streptococcus mutans and streptococcus mitis in Vitro. J Baghdad Coll Dent. 2012;24(3):154-7. [Link]
4. Kooti W, Hasanzadeh Noohi Z, Sharafi-Ahvazi N, Asadi-Samani M, Ashtary Larky D. Phytochemistry, pharmacology, and therapeutic uses of black seed (Nigella sativa). Chin J Nat Med. 2016;14(10):732-45. [Link] [DOI:10.1016/S1875-5364(16)30088-7]
5. Maciel MAM, Pinto C A, Veiga Jr VF, Grynberg NF, Echevarria A. [Medicinal plants: A need for multidisciplinary studies]. Química Nova. 2002;25(3):429-38. [Link] [DOI:10.1590/S0100-40422002000300016]
6. Maria Jancy Rani P, Kannan PSM, Kumaravel S. GC-MS analysis of Lantana camara L leaves. Int J Pharm Res Dev. 2011;2(11):63- 66. [Link]
7. Hadjzadeh MR, Keshavarzi Z, Tabatabaee Yazdi SA, Ghasem SM, Rajaei Z, Khajavi Rad A. Effect of alcoholic extract of Nigella sativa on cisplatin-induced toxicity in rat. Iran J Kidney Dis. 2012;6(2):99-104. [Persian] [Link]
8. Khare CP. Encyclopedia of Indian medicinal plants. Berlin Heidelberg: Springer Verlag New York; 2004. [Link]
9. Sawarkar SP, Verma H, Deshmukh PV. Comparative study of antimicrobial effect of nigella sativa seed extracts from different geographies. Int J Pharmacogn. 2016;3(6):257-64. [Link]
10. Aljabre SHM, Alakloby OM, Randhawa MA. Dermatological effects of Nigella sativa. J Dermatol & Dermatol Surg. 2015;19(2):92-98. [Link] [DOI:10.1016/j.jdds.2015.04.002]
11. Paarakh PM. Nigella sativa Linn.-A comprehensive review. Indian J Nat Prod Resour. 2010;1(4):409-29. [Link]
12. Al-Jassir MS. Chemical composition and microflora of black cumin (Nigella sativa L) seeds growing in Saudi Arabia. Food Chem. 1999;45(4):239-42. [Link] [DOI:10.1016/0308-8146(92)90153-S]
13. choudhary MI, E-shahwar D, Parveen Z, Jabbar A, Ali I, ur-Rahman A. Antifungal steroidal lactones from withania coagulance. phytochem. 1995;40(4):1243-6. [Link] [DOI:10.1016/0031-9422(95)00429-B]
14. Nickavar B, Mojab F, Javidnia K, Roodgar Amoli MA. Chemical composition of the fixed and volatile oils of Nigella sativa L. from Iran. Z Naturforsch C J Biosci. 2003;58(9-10):629-31. [Link] [DOI:10.1515/znc-2003-9-1004] [PMID]
15. Parekh J, Karathia N, Chanda S. Screening of some traditionally used medicinal plants for potential antibacterial activity. Indian J Pharm Sci. 2006;68(6):832-34. [Link] [DOI:10.4103/0250-474X.31031]
16. Roy J, Shaklega DM, Callery PS, Thomas JG. Chemical constituents and antimicrobial activity of a traditional herbal medicine containing garlic and black cumin. African J Tradit, Complement Altern Med. 2006;3(2):1-7. [Link] [DOI:10.4314/ajtcam.v3i2.31151]
17. Ali BH, Blunden G. Pharmacological and toxicological properties of Nigella sativa. Phytother Res. 2003;17(4):299-305. [Link] [DOI:10.1002/ptr.1309] [PMID]
18. Morsi NM. Antimicrobial effect of crude extracts of Nigella sativa on multiple antibiotics-resistant bacteria. J Acta Microbiol Pol. 2000;49(1):63-74. [Link]
19. Ahmad I, Muneer KM, Tamimi IA, Chang ME, Ata MO, Yusuf N. Thymoquinone suppresses metastasis of melanoma cells by inhibition of NLRP3 inflammasome. Toxicol Appl Pharmacol. 2013;270(1):70-6. [Link] [DOI:10.1016/j.taap.2013.03.027] [PMID]
20. Yuan T, Nahar P, Sharma M, Liu K, Slitt A, Aisa HA et al. Indazole-type alkaloids from Nigella sativa seeds exhibit antihyperglycemic effects via AMPK activation in vitro. J Nat Prod. 2014;77(10):2316-20. [Link] [DOI:10.1021/np500398m] [PMID] [PMCID]
21. Harzallah H J, Noumi E, Bakhrouf A, Bekir K, Mahjoub T. Chemical composition, antibacterial and antifungal properties of Tunisian Nigella sativa fixed oil. Afr J Microbiol Res. 2012;6(22):4675-9. [Link] [DOI:10.5897/AJMR11.1073]
22. Arafa ESA, Zhu Q, Shah ZI, Wani G, Barakat BM, Racoma I et al. Thymoquinone up-regulates PTEN expression and induces apoptosis in doxorubicin-resistant human breast cancer cells. Mutat Res. 2011;10(1-2):28-35. [Link] [DOI:10.1016/j.mrfmmm.2010.10.007] [PMID] [PMCID]
23. Khader M, Bresgen N, Eckl PM. In vitro toxicological properties of thymoquinone. Food Chem Toxicol. 2009;47(1):129-33. [Link] [DOI:10.1016/j.fct.2008.10.019] [PMID]
24. Vahdati Mashhadian N, Rakhshandeh H, Omidi A. An investigation on LD50 and subacute hepatic toxicity of Nigella sativa seed extracts in mice. Pharmazie. 2005;60(7):544-7. [Link]
25. Mansour MA, Ginawi OT, El-Hadiyah T, El-Khatib AS, Al-Shabanah OA, Al-Sawaf HA. Effects of volatile oil constituents of Nigella sativa on carbon tetrachloride-induced hepatotoxicity in mice: evidence for antioxidant effects of thymoquinone. Res Commun Mol Pathol Pharmacol. 2001;110(3-4):239-51. [Link]
26. Al-Ali A, Alkhawajah AA, Randhawa MA, Shaikh NA. Oral and intraperitoneal LD50 of thymoquinone, an active principle of Nigella sativa, in mice and rats. J Ayub Med Coll Abbottabad. 2008;20(2):25-7. [Link]
27. Perera WPRT, Liyanage JA, Dissanayake KGC, Chandrasiri WAL, Gunathilaka H. A review on pharmacological activities and anti-microbial properties of Nigella sativa and isolated Thymoquinone. J Med Plants Stud. 2021;9(3):118-22. [Link] [DOI:10.22271/plants.2021.v9.i3b.1277]
28. Toppozada HH, Mazloum H, El-Dakhakhny M. The antibacterial properties of the Nigella sativa l. seeds. Active principle with some clinical applications. J Egypt Med Assoc. 1965;48 suppl:187-202. [Link]
29. El-Fatatry HM. Isolation and structure assignment of an antimicrobial principle from the volatile oil of Nigella sativa L. seeds. Pharmazie. 1975;30(2):109-11. [Link]
30. Kamal A , Arif JM, Ahmad I. Potential of Nigella sativa L. seed during different phases of germination on inhibition of bacterial growth. J Biotechnol Pharm Res. 2010;1(1):009-13. [Link]
31. Abu-Al-Basel MA. In vitro and In vivo Anti-Microbial Effects of Nigella sativa Linn. Seed Extracts Against Clinical Isolates from Skin Wound Infections. American j Appl Sci. 2009;6(8):1440-47. [Link] [DOI:10.3844/ajassp.2009.1440.1447]
32. Namjoo A, Sadri SM, Rafeian M, Ashrafi K, Shahin Fard N, Ansari samani R et al . Comparing the effects of Nigella sativa extract and gentamicin in treatment of urinary tract infection caused by Ecoli. J Mazandaran Univ Med Sci. 2013;22(96):22-9. [Persian] [Link]
33. Gharibi D, Ghorbanpoor Najafabadi M, Mohabat A. Study Of Antibacterial Activity Of Ethanol Extract From Nigella Sativa Against Some Important Veterinary Bacterial Pathogens. J Vet Microbiol. 2012;8(1):13-21. [Persian] [Link]
34. Bita A, Rosu A, Calina D, Rosu L, Zlatian O, Dindere C et al. An alternative treatment for candida infections with Nigella sativa extracts. Eur J Hosp Pharm: Sci Pract. 2012;19(2):162. [Link] [DOI:10.1136/ejhpharm-2012-000074.203]
35. Shenawy NS, Soliman MFM, Reyad SI. The effect of antioxidant properties of aqueous garlic extract and Nigella sativa as anti-schistosomiasis agents in mice. Rev Inst Med Trop São Paulo. 2008;50(1):29-36. [Link] [DOI:10.1590/S0036-46652008000100007] [PMID]
36. Hindi NKK, Al-Mahdi ZKA, Chabuck ZAG. Antibacterial activity of the aquatic extract of fresh, Dry powder ginger and crud oil of ginger (Zingiber officinale) against different types of bacteria in Hilla city, Iraq. Int J Pharm Pharm Sci. 2014;6(5):414-17. [Link]
37. Forbes BA, Sahm DF, Weissfeld AS. Bailey and Scott's diagnostic microbiology. Edition 12th. Amsterdam: Elsevier;2007 [Link]
38. Salman MT, Khan RA, Shukla I. Antimicrobial activity of Nigella sativa Linn. seed oil against multi-drug resistant bacteria from clinical isolates. Indian J Nat Prod Resour. 2008;7(1):10-14. [Link]
39. Hanafy MS, Hatem ME. Studies on the antimicrobial activity of Nigella sativa seed (black cumin). J Ethnopharmacol. 1991;34(2-3):275-8. [Link] [DOI:10.1016/0378-8741(91)90047-H]
40. Sokmen A, Jones BM, Erturk M. The in vitro antibacterial activity of Turkish medicinal plants. J Ethnopharmacol. 1999;67(1):79-86. [Link] [DOI:10.1016/S0378-8741(98)00189-5]
41. Al-Ameedy TH, Omran R. Antimicrobial Activity of Nigella Sativa Extract Against some Bacterial and Fungal Species. J Univ Babylon, Pure Appl Sci. 2019;27(1):277-86. [Link]
42. Rafati S, Niakan M, Naseri M. Anti-microbial effect of Nigella sativa seed extract against staphylococcal skin Infection. Med J Islam Repub Iran. 2014;28(1):261-65. [Link]
43. Zuridah H, Fairuz ARM, Zakri AHZ, Rahim MNA. In vitro antibacterial activity of Nigella sativa Against Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli, and Bacillus cereus. Asian J Plant Sci. 2008;7(3):331-3. [Link] [DOI:10.3923/ajps.2008.331.333]
44. Hosseinzadeh H, Fazly Bazzaz BS, Motevaly Haghi M. Antibacterial Activity of Total Extracts and Essential oil of Nigella Sativa L. Seeds in Mice. Pharmacologyonline. 2007;2:429-435 [Link]
45. Fico G, Panizzi L, Flamini G, Braca A, Morelli I, Tomè F, Cioni PL. Biological Screening of Nigella damascena for Antimicrobial and Molluscicidal Activities. Phytother Res. 2004;18(6):468-70. [Link] [DOI:10.1002/ptr.1454] [PMID]
46. Abdallah EM. Black seed (Nigella sativa) as antimicrobial drug a mini-review. Nov Approach Drug Des Dev. 2017;3(2):9-13. [Link]