other تجهیزات پزشکی برای کمک به جانبازان يا معلولان Assistive Medical Device for Veterans or Handicapped توصیفی و نظرسنجی Descriptive & Survey <span style="font-size:12pt"><span style="line-height:150%"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:10.0pt"><span style="line-height:150%"><span cambria="" style="font-family:">Background:</span></span></span></b><span style="font-size:10.0pt"><span style="line-height:150%"><span cambria="" style="font-family:"> Although anti-double-stranded DNA (dsDNA) antibodies is diagnostic markers for systemic lupus erythematosus (SLE), their limitations necessitate the identification of supplementary biologically relevant biomarkers to enhance diagnostic accuracy.</span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:150%"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:10.0pt"><span style="line-height:150%"><span cambria="" style="font-family:">Objective:</span></span></span></b><span style="font-size:10.0pt"><span style="line-height:150%"><span cambria="" style="font-family:"> To evaluate serum levels of mitogen-activated protein kinase 4 (MAPK4) and extracellular signal-regulated kinase 5 (ERK5) in patients with active SLE and determine their individual and combined diagnostic performance relative to anti-dsDNA antibodies.</span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:150%"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:10.0pt"><span style="line-height:150%"><span cambria="" style="font-family:">Patients and methods:</span></span></span></b><span style="font-size:10.0pt"><span style="line-height:150%"><span cambria="" style="font-family:"> This study involved 119 women with active SLE and 61 healthy female controls. Serum concentrations of MAPK4, ERK5 and the anti-dsDNA index were quantified using enzyme-linked immunosorbent assays (ELISA). Diagnostic performance was analyzed using receiver operating characteristic (ROC) curves, principal component analysis (PCA), and multivariate logistic regression.</span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:150%"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:10.0pt"><span style="line-height:150%"><span cambria="" style="font-family:">Results:</span></span></span></b><span style="font-size:10.0pt"><span style="line-height:150%"><span cambria="" style="font-family:">&ensp;SLE patients showed a significant increase in MAPK4, ERK</span></span></span><span style="font-size:10.0pt"><span style="line-height:150%"><span cambria="" style="font-family:">5 and anti-</span></span></span><span style="font-size:10.0pt"><span style="line-height:150%"><span cambria="" style="font-family:">ds DNA levels compared with the controls (all</span></span></span><span style="font-size:10.0pt"><span style="line-height:150%"><span cambria="" style="font-family:"> P < 0.001). The ROC curves for all have AUCs of 0.99.</span></span></span><span style="font-size:10.0pt"><span style="line-height:150%"><span cambria="" style="font-family:"> PCA demonstrated that the three biomarkers loaded onto a single dominant component that explained most of the variance. A logistic model&ensp;combining the three markers achieved classification accuracy</span></span></span><span style="font-size:10.0pt"><span style="line-height:150%"><span cambria="" style="font-family:"> of 99.4 %. </span></span></span><span style="font-size:10.0pt"><span style="line-height:150%"><span cambria="" style="font-family:">The three biomarkers showed consistent behavior within the composite statistical model between them, suggesting that they are involved in a shared immunological pathway.</span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:150%"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:10.0pt"><span style="line-height:150%"><span cambria="" style="font-family:">Conclusion:</span></span></span></b><span style="font-size:10.0pt"><span style="line-height:150%"><span cambria="" style="font-family:"> Serum MAPK4 and ERK5 levels were significantly elevated in active SLE, demonstrating exceptional diagnostic performance comparable to </span></span></span><span style="font-size:10.0pt"><span style="line-height:150%"><span cambria="" style="font-family:">that of anti-dsDNA antibodies. Integrating these novel kinases into a multi-marker serological panel substantially enhances the diagnostic capability for SLE, particularly when conventional mar</span></span></span><span style="font-size:10.0pt"><span style="line-height:150%"><span cambria="" style="font-family:">kers yield inconclusive results.</span></span></span></span></span></span><br> &nbsp; 1001 1015 http://ijwph.ir/browse.php?a_code=A-10-2454-1&slc_lang=other&sid=1 G.H. Abdulhadi ghid.h.ah@comed.uobaghdad.edu.iq 15300319475328460029281 15300319475328460029281 Yes